The guide also explains what cryo-EM measures on each particle, how each structural readout relates to encapsulation and in-vivo expression, and where the relationship reaches its current limits.
Evaluated effects of heat, freeze-thaw cycling, and mechanical stress on commercially available COVID-19 Vaccine LNP material. Cryo-EM structural data was recorded.
Evaluated influence of two mixing technologies on identical base formulations. Cryo-EM structural data, mRNA encapsulation efficiency (EE), DLS size, and in vivo luciferase expression in a mouse model were recorded.
Evaluated effects of stress and other factors on identical base formulations: mechanical and thermal stress, prolonged ethanol exposure, mRNA loading, and pumping system. Cryo-EM structural data, mRNA EE, and in vivo luciferase expression in a mouse model were recorded.
Varied synthesis pH, payload concentration, buffer, and residence time. Cryo-EM structural data and EE were measured at five in-process controls
For professionals working on LNP formulation and development, including:
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