Epitope Mapping

High-throughput cryo-EM platform 3D structural epitope and paratope mapping

3D Epitope Mapping by Cryo-EM

High-Throughput Cryo-EM Platform for Epitope Discovery

Unlock the full potential of epitope mapping with our unique, high-throughput cryo-EM platform. Specializing in the visualization of challenging 3D epitopes, our service enables the screening of multiple FAB/mAbs complexes. This allows for the detailed examination of conformations, contacting residues, and binding locations, crucial for identifying non-linear epitopes that traditional methods often miss. Our approach opens new possibilities for structural information, paving the way for innovative antibody development and effective drug design.

Tailored Comprehensive Cryo-EM Services

Our state-of-the-art cryo-electron microscopy (cryo-EM) services offer a fully integrated solution for protein structure determination. Combining advanced wet lab techniques with high-performance computing infrastructure, we ensure efficient processing of samples through a structured three-phase process:

  • Phase 1: Pilot Study – We begin with a pilot study to assess the feasibility of target proteins, setting the foundation for more detailed structural analysis.
  • Phase 2: Optimization – Following a successful pilot, we proceed to optimization, focusing on cryo-grid refinement to enhance data quality.
  • Phase 3: 3D Structure Determination – The final phase involves detailed 3D structure determination, utilizing optimized data for comprehensive analysis.

Streamlined Project Management

Each project kicks off with a strategic meeting to outline objectives and timelines, ensuring alignment with your goals. Regular updates and alignment meetings keep you informed of progress, with timelines tailored to each project’s unique requirements. Our integrated workflow from lab to computational analysis not only guarantees precise, high-quality results but also advances the frontiers of structural biology research, enabling rapid responses to global health challenges.

Our combined expertise in epitope mapping and structural determination underscores our commitment to delivering cutting-edge solutions, supporting your journey from complex biological questions to clear structural insights.

Advance your drug design
Screen multiple FABs
Elucidate confirmational epitopes
3D epitope mapping High-throughput structural screening Discover FAB binding sites Determine contacting residues Unlock the full potential of epitope mapping
Application High-throughput FAB screening Antibody development Augmenting lead candidate selection Elucidate confirmational epitopes

Gain access to structural epitope & paratope information of your complexes

Our 3D Epitope Mapping

for high-resolution
01

Feasibility study

Sample evaluation

Negative stain screening
02

Screening camapaign

With an optimized workflow, we collect data for low-resolution structures of each target-FAB complex resolution structures of each target-FAB complex of each
03

Data collection and processing

Additional data collection for high-resolution on state-of-the-art-cryo EM hardware

Processing by cryo-EM experts
04

Results

Final sample report

PDB file

Structure map

Sample requirements

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We prepare everything else. We receive your sample – optimize and test it, before returning the data in a personalized way that suits you.

Sample amounts
Get insight into detailed parameters with sample amounts of only 30-50 μl (>0.5 mg/ml).
Turnaround times
Get first results within 2 to 4 weeks. Depending on number of compounds.
Results
Receive a 3D structure together with a ready-to-use PDB file, including an extensive report.