Plasmid
DNA analysis

INVESTIGATIVE WORK WHERE COMMON METHODS FAIL

Leave your samples in professional hands with our electron microscopy plasmid DNA analysis.

Traditional gel electrophoresis can end with ambiguous results, and omits details such as replication. TEM DNA analysis is the ultimate magnifying glass to determine a range of conditions in research that requires a complete understanding of sample behavior.

Visualize plasmid conformation, such as supercoiling, and replication forks, and differentiate double-and single-stranded DNA. Gain insight into distribution and plasmid length measurements at single molecule levels – all combined in a comprehensive report prepared by our TEM experts.

Our plasmid analysis is based on established methods and tailored to individual demands.

One analysis—multiple features

  • Sample content & contamination
  • Morphology (Replication forks & junctions)
  • Distribution (i.e. linear vs. circular)
  • 3D conformations (e.g. Supercoiled or interlocked plasmids)

Key application areas

  • R&D
  • Microbiology
  • Plasmid production/manufacturing

Application examples

  • Quality control
  • Troubleshooting (unexpected results)
  • Morphological plasmid characterization

AT THE FOREFRONT OF
INDUSTRIAL CRYO-EM TECHNOLOGY

LET'S DISCUSS YOUR PROJECT

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Plasmid DNA analysis
Quick & extensive

Sample preparation

  • We receive the sample and prepare it for TEM measurements
01

Data Collection

  • High-throughput data collection on state-of-the-art EM hardware
02

Data analysis

  • We analyse the data, measuring plasmid lengths, morphology and distribution
03

Results

  • Detailed report on sample content together with statistical information
04

Sample requirements

  • Purified DNA samples.
  • A list of buffer conditions and any additional information that may be useful (Agarose-gels etc.). The more information available to us, the faster we can evaluate the project.

We prepare everything else! We receive your sample – optimize and test it, before returning the data in a personalized way that suits you.

Sample amounts

Get insight into detailed parameters with sample amounts of only 30-50 μl.

Turnaround times

First results within 20 days.

Results

Receive an extensive, personalized report on plasmid parameters.

Let's discuss your project

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