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We generate precise 3D molecular blueprints of your Antigen-Antibody complex to enable detailed insights for advanced research and development.
Evaluate an entire lead series to enable more informed decision-making.
Lead Series
10's of candidates
ATEM offers two analysis options to meet your budget and timeline requirements.
Ideal for rational lead candidate selection AI model validation.
Ideal for high-end Mode of Action studies, lead optimization, IND filings, & patent applications.
We recognize that every client has unique needs and preferences. Explore our dedicated options to make your project benefit from readily available Gold Standard 3D Epitope Mapping data.
We ensure that your project is completed on time, within budget, and to your highest satisfaction. Here’s a step-by-step overview of how our process works.
From intermetiate resolutions and higher, it is possible to distinguish the binding area of the antibody on the protein. The specificity of binding area details increases with higher resolutions.
At high resolutions (under 4 Å) it is possible to distinguish separate amino acids and confirm precise binding areas. At lower resolutions, mostly larger amino acids are distinguishable.
By comparing different 3D structures of protein-antibody complexes, it is possible to see whether antibodies sterically clash.
Some antibodies may induce conformational changes within protein structures. 3D structural information can specify where, and what changes occur.
Antibody binding may affect multimerization, for example inhibiting dimerization. With 3D structure information, these disruptions are spotted.
Required sample amount*
A minimum of 0.5 mg protein, 0.5 mg Fab or 1 mg antibody.
We accept all formats, however, IgGs can be difficult to fully resolve in 3D. Therefore, we recommend FABs, and also offer FAB isolation from IgG antibodies.
Cryo-EM is possible for investigating PROTAC complexes. For optimal results, it requires stable formation of preferably large complexes.
The Pilot Phase quickly assesses project viability for continuation to intermediate resolutions. In some cases, the project requires additional biochemical work to increase stability or orientations of the sample on cryo-grids.
Yes, cryo-EM works for membrane proteins in both detergent and reconstituted conditions.
Our experts are always happy to discuss how we can support you with our cryo-EM-focused solutions. Schedule a call with us.
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