Adeno-associated virus-like particles (AAV) have become a popular method of gene delivery, paving the way for gene therapies, including CRISPR/Cas9 editing.
Unfortunately, not all viral particles are correctly packed after production, lacking the important DNA payload. Accurately determining the capsid “fill-ratio” of viral vector delivery systems is thus essential for the development and manufacture of safe and reliable drug products.
ATEM’s cryo-EM technology gives you statistically valid, highly accurate insights into the full and empty capsid ratio of your sample with single-particle precision. Not only that – we can show you the fraction of partially filled particles in your sample.
We ensure the most reliable results for your project through rapid turnaround times and the highest quality standards under GMP regulations.
Quantify empty AAV particles
Filled AAV particles
Intermediates between empty and filled.
We successfully developed a method that allows us to distinguish between empty/intermediate/full capsids. You can read the full version of our case study here.
We prepare everything else! We receive your sample – optimize and test it, before returning the data in a personalized way that suits you.
Get insight into detailed parameters with sample amounts of only 30-50 μl.
First results in 20 days or faster.
Receive an extensive report, custom made to highlight areas of interest.